Proteomic Biology Using LC/MS Large Scale Analysis of Cellular Dynamics and Function
by Takahashi, Nobuhiro; Isobe, Toshiaki; Desiderio, Dominic M.; Nibbering, Nico M.Edition: 1st
Format: Hardcover
Pub. Date: 2007-11-02
Publisher(s): Wiley-Interscience
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Summary
This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.
Author Biography
Nobuhiro Takahashi, PhD, is a Professor of Applied Biological Science in the Department of Agriculture at Tokyo University of Agriculture and Technology.
Toshiaki Isobe, PhD, is a Professor of Biochemistry in the Department of Chemistry at Tokyo Metropolitan University.
Table of Contents
| Introduction | p. vii |
| Overview of Proteomics | p. 1 |
| What Is Proteomics? | p. 1 |
| Establishment of Proteomics Using Mass Spectrometry | p. 3 |
| Protein Separations by Two-Dimensional Electrophoresis | p. 3 |
| Development of the Technologies for Protein Identification | p. 5 |
| Protein Identification Based on Gel Separation and Mass Spectrometry | p. 6 |
| Strategies for Characterizing Proteomes and Understanding Proteome Function | p. 15 |
| Modification-Specific Proteomics | p. 17 |
| Activity-Based Protein Profiling/Enzyme Substrate Proteomics | p. 31 |
| Subcellular (Organellar) Proteomics | p. 41 |
| Machinery or Complex Interaction Proteomics | p. 45 |
| Dynamic Proteomics | p. 46 |
| References | p. 49 |
| Proteomic Tools for Analysis of Cellular Dynamics | p. 63 |
| LC-Based Proteomics Technologies | p. 63 |
| LC-MS system for Peptide Separation and Identification | p. 71 |
| Application of LC-MS Methods to Functional Proteomics | p. 101 |
| Development of Quantitative Proteomics | p. 131 |
| Isotope Labeling for Quantitative Analysis Using MS | p. 132 |
| Quantification Strategy for LC-MS Analysis of Isotope-Labeled Peptide Mixture and Software for Computer Analysis | p. 140 |
| Label-Free Quantification Software | p. 145 |
| Absolute Quantification | p. 147 |
| References | p. 153 |
| Dynamics of Functional Cellular Machinery: From Statics to Dynamics in Proteomic Biology | p. 167 |
| Dynamic Analysis of Cellular Function | p. 167 |
| Strategy for Dynamic Analysis of Cellular Machinery (Multiprotein Complexes) | p. 168 |
| Methods for the Isolation of a Cellular Machine/Multiprotein Complex | p. 174 |
| Cellular Machinery (Multiprotein Complex) | p. 196 |
| Dynamics of Ribosome Biogenesis | p. 198 |
| Snapshot Analysis of Preribosomal Particles in Yeast | p. 201 |
| Snapshot Analysis of Preribosomal Particles in Mammals | p. 207 |
| Quantitative (Dynamic) Analysis Using Isotope-Labeled Reagents | p. 224 |
| Outline of Human/Mammalian Ribosome Biogenesis | p. 231 |
| Dynamic Analysis of Subcellular Structures | p. 232 |
| Proteome Dynamics of the Nucleolus | p. 233 |
| References | p. 236 |
| Index | p. 249 |
| Table of Contents provided by Ingram. All Rights Reserved. |
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